American Society for Clinical Pathology (ASCP) Histotechnologist Practice Test

Which stain is known as the most sensitive of the lipid dyes used in hematopathology?

1. Oil Red O

2. Sudan black B

3. Fat red 7B

4. Neutral lipid stain

The correct answer is: Sudan black B

Sudan Black B is recognized as the most sensitive lipid dye utilized in hematopathology. This stain is particularly effective in highlighting lipids due to its ability to bind to both neutral lipids and phospholipids. Its sensitivity allows for the detection of even minor amounts of lipid within tissue sections, which is crucial in diagnosing certain hematological disorders, including leukemias and other neoplasms where lipid accumulation may occur. The versatility of Sudan Black B stems from its specificity for lipid components, enabling pathologists to differentiate between normal and abnormal lipid storage. When combined with other staining methods, it can enhance the assessment of cellular morphology and pathology related to lipid metabolism. Other stains may also have important applications but tend not to exhibit the same sensitivity as Sudan Black B. For instance, while Oil Red O is an excellent stain for neutral lipids, it is often less sensitive in certain contexts compared to Sudan Black B. Thus, the use of Sudan Black B in clinical practice is preferred for cases requiring precise lipid identification.